[Histonet] RE: Masson's Trichrome

[Jonathan Cremer]

The PMA-PTA can be reused, even when the color changes to green. I don't see a problem in using fresh 1% acetic acid with every batch of slides? I just make up a 1l bottle, or mix it quickly when doing the stain. Glacial AA isn't that expensive, and it's no problem to pour down the drain (household vinegar is more concentrated). Unless you have to hang on to every single molecule of dye stuff and have it hauled off by a waste company...
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Jonathan Cremer
Laboratory Technician
TARGID - KU Leuven

Gasthuisberg CDG; Labo Experimentele Immunologie; Herestraat 49 bus 811; 3000 Leuven; Belgium

________________________________________
Van: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] namens Berger, Rebecca [BergerR1 <@t> email.chop.edu]
Verzonden: maandag 7 oktober 2013 15:53
Aan: histonet <@t> lists.utsouthwestern.edu
Onderwerp: [Histonet] Masson's Trichrome

Hi Histonet,
Quick question about Masson's Trichrome. I'm staining several batches of slides by hand, and normally only use the phosphotungstic/phosphomolybdic acid solution, as well as the 1% acetic acid solution, just once and replace it each time I do a stain.  Does anyone out there re-use the acid solution? It seems like a real waste (in many ways) to replace it each time I do the stain but I've yet to experiment with re-using them.
Thanks for your help in advance,
Becky


Rebecca Berger, HT(ASCP)CM
Research Technician
Division of Orthopedic Surgery
The Children's Hospital of Philadelphia Research Institute
3615 Civic Center Boulevard, ARC904 (Lab)
Philadelphia, PA 19104
Phone: 267-425-2076  Email: bergerr1 <@t> email.chop.edu<mailto:bergerr1 <@t> email.chop.edu>
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