AW: [Histonet] HT HistoDeck question...

[Gudrun Lang]

We use 36-40% formaldehyde for a minimal time of 2-3 dips for fast frozen
HE. On the other side the slides stand in the solution as long as all
frozens are already cut.
Commercial formaldehyde contents a good part of methanol (a MSDS says
5-15%). So fast fixation is a combination of formaldehyde and alcohol
fixation.
Additional we use Harris hematoxylin, that's also mainly made of ethanol. 
So in my opinion all together fixation is rather due to alcohol in this way.


Gudrun 
 

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Rathborne,
Toni
Gesendet: Donnerstag, 03. Oktober 2013 20:34
An: 'Jennifer MacDonald'; Grantham, Andrea L - (algranth)
Cc: HISTONET; histonet-bounces <@t> lists.utsouthwestern.edu
Betreff: RE: [Histonet] HT HistoDeck question...

For those of you who use the 40% formaldehyde, how long is you fixation time
on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes it gets
hectic if you have multiple frozens all at once. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Thursday, October 03, 2013 2:04 PM
To: Grantham, Andrea L - (algranth)
Cc: HISTONET; histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...

We also use this oil red O method and use the 40% formaldehyde.
The questions lacks enough information to correctly answer it.  I am sure
the author of the question had something in mind and other options didn't
occur to him/her at the time.
Jennifer




From:   "Grantham, Andrea L - (algranth)" <algranth <@t> email.arizona.edu>
To: 
Cc:     HISTONET <histonet <@t> lists.utsouthwestern.edu>
Date:   10/03/2013 08:33 AM
Subject:        Re: [Histonet] HT HistoDeck question...
Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu



I'd go with "A", but it really depends on what you are going to do with the
sections after fixation.

In the protocol for Oil Red O in Freida's second edition (that I use almost
daily combined with some steps from PolyScientific's ORO protocol), step #1
says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in
the liquid 40%.
I have fixed frozen sections for regular H&E and other stains as well in 40%
formaldehyde and they come out beautiful.
Unless there is some specific request or reason to use cold acetone or
alcohol this is what I use.

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097





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