[Histonet] HT HistoDeck question...

[Rathborne, Toni]

For those of you who use the 40% formaldehyde, how long is you fixation time on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes it gets hectic if you have multiple frozens all at once. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald
Sent: Thursday, October 03, 2013 2:04 PM
To: Grantham, Andrea L - (algranth)
Cc: HISTONET; histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...

We also use this oil red O method and use the 40% formaldehyde.
The questions lacks enough information to correctly answer it.  I am sure the author of the question had something in mind and other options didn't occur to him/her at the time.
Jennifer




From:   "Grantham, Andrea L - (algranth)" <algranth <@t> email.arizona.edu>
To: 
Cc:     HISTONET <histonet <@t> lists.utsouthwestern.edu>
Date:   10/03/2013 08:33 AM
Subject:        Re: [Histonet] HT HistoDeck question...
Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu



I'd go with "A", but it really depends on what you are going to do with the sections after fixation.

In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%.
I have fixed frozen sections for regular H&E and other stains as well in 40% formaldehyde and they come out beautiful.
Unless there is some specific request or reason to use cold acetone or alcohol this is what I use.

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097





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