[Histonet] HT HistoDeck question...
Jennifer MacDonald
JMacDonald <@t> mtsac.edu
Thu Oct 3 16:53:59 CDT 2013
the protocol calls for 1 minute in the 40% formaldehyde and then rinse the
sections well.
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
To: "'Jennifer MacDonald'" <JMacDonald <@t> mtsac.edu>, "Grantham, Andrea L
- (algranth)" <algranth <@t> email.arizona.edu>
Cc: HISTONET <histonet <@t> lists.utsouthwestern.edu>,
"histonet-bounces <@t> lists.utsouthwestern.edu"
<histonet-bounces <@t> lists.utsouthwestern.edu>
Date: 10/03/2013 11:42 AM
Subject: RE: [Histonet] HT HistoDeck question...
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
For those of you who use the 40% formaldehyde, how long is you fixation
time on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes
it gets hectic if you have multiple frozens all at once.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Thursday, October 03, 2013 2:04 PM
To: Grantham, Andrea L - (algranth)
Cc: HISTONET; histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...
We also use this oil red O method and use the 40% formaldehyde.
The questions lacks enough information to correctly answer it. I am sure
the author of the question had something in mind and other options didn't
occur to him/her at the time.
Jennifer
From: "Grantham, Andrea L - (algranth)" <algranth <@t> email.arizona.edu>
To:
Cc: HISTONET <histonet <@t> lists.utsouthwestern.edu>
Date: 10/03/2013 08:33 AM
Subject: Re: [Histonet] HT HistoDeck question...
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
I'd go with "A", but it really depends on what you are going to do with
the sections after fixation.
In the protocol for Oil Red O in Freida's second edition (that I use
almost daily combined with some steps from PolyScientific's ORO protocol),
step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the
slides in the liquid 40%.
I have fixed frozen sections for regular H&E and other stains as well in
40% formaldehyde and they come out beautiful.
Unless there is some specific request or reason to use cold acetone or
alcohol this is what I use.
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724
algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415 Fax: 520.626.2097
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