[Histonet] HT HistoDeck question...

[Jennifer MacDonald]

the protocol calls for 1 minute in the 40% formaldehyde and then rinse the 
sections well.



From:   "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
To:     "'Jennifer MacDonald'" <JMacDonald <@t> mtsac.edu>, "Grantham, Andrea L 
-       (algranth)" <algranth <@t> email.arizona.edu>
Cc:     HISTONET <histonet <@t> lists.utsouthwestern.edu>, 
"histonet-bounces <@t> lists.utsouthwestern.edu" 
<histonet-bounces <@t> lists.utsouthwestern.edu>
Date:   10/03/2013 11:42 AM
Subject:        RE: [Histonet] HT HistoDeck question...
Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu



For those of you who use the 40% formaldehyde, how long is you fixation 
time on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes 
it gets hectic if you have multiple frozens all at once. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Thursday, October 03, 2013 2:04 PM
To: Grantham, Andrea L - (algranth)
Cc: HISTONET; histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...

We also use this oil red O method and use the 40% formaldehyde.
The questions lacks enough information to correctly answer it.  I am sure 
the author of the question had something in mind and other options didn't 
occur to him/her at the time.
Jennifer




From:   "Grantham, Andrea L - (algranth)" <algranth <@t> email.arizona.edu>
To: 
Cc:     HISTONET <histonet <@t> lists.utsouthwestern.edu>
Date:   10/03/2013 08:33 AM
Subject:        Re: [Histonet] HT HistoDeck question...
Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu



I'd go with "A", but it really depends on what you are going to do with 
the sections after fixation.

In the protocol for Oil Red O in Freida's second edition (that I use 
almost daily combined with some steps from PolyScientific's ORO protocol), 
step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the 
slides in the liquid 40%.
I have fixed frozen sections for regular H&E and other stains as well in 
40% formaldehyde and they come out beautiful.
Unless there is some specific request or reason to use cold acetone or 
alcohol this is what I use.

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097





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