[Histonet] HT HistoDeck question...

[Jennifer MacDonald]

We also use this oil red O method and use the 40% formaldehyde.
The questions lacks enough information to correctly answer it.  I am sure 
the author of the question had something in mind and other options didn't 
occur to him/her at the time.
Jennifer




From:   "Grantham, Andrea L - (algranth)" <algranth <@t> email.arizona.edu>
To: 
Cc:     HISTONET <histonet <@t> lists.utsouthwestern.edu>
Date:   10/03/2013 08:33 AM
Subject:        Re: [Histonet] HT HistoDeck question...
Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu



I'd go with "A", but it really depends on what you are going to do with 
the sections after fixation.

In the protocol for Oil Red O in Freida's second edition (that I use 
almost daily combined with some steps from PolyScientific's ORO protocol), 
step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the 
slides in the liquid 40%.
I have fixed frozen sections for regular H&E and other stains as well in 
40% formaldehyde and they come out beautiful.
Unless there is some specific request or reason to use cold acetone or 
alcohol this is what I use.

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097





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