[Histonet] HT HistoDeck question...

[Grantham, Andrea L - (algranth)]

I'd go with "A", but it really depends on what you are going to do with the sections after fixation.

In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%.
I have fixed frozen sections for regular H&E and other stains as well in 40% formaldehyde and they come out beautiful.
Unless there is some specific request or reason to use cold acetone or alcohol this is what I use.

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097