[Histonet] TIA1 and granzyme B

[avistarop <@t> ffyb.uba.ar]

Thank you for information.
I will Contact you if I have a doubt.
 Thank you so much!!!!

Aldana


> We are in the completing our validation for both TIA-1 and Granzyme B.
> Here's what we are using:
>
> TIA (EP243) Rabbit Monoclonal from Cell Marque (Predilute, ready to use)
> Protocol for Ventana Ultra is: Extended Retrieval CC1 (64 minutes),
> antibody incubation 40 minutes, at 37 degrees with amplification.
>
> Granzyme B (Polyclonal) from Cell Marque Catalog#760-4283
> Protocol for Ventana Ultra: Mild CC1, at 37 degrees, antibody incubation
> 16 minutes.
>
> Hope that helps!
> Happy Thanksgiving
>
> Cecilia M. Obregon
> Memorial Regional Hospital
> 3501 Johnson Street
> Hollywood, FL 33021
> Ph: 954-265-5317
>
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of
> avistarop <@t> ffyb.uba.ar [avistarop <@t> ffyb.uba.ar]
> Sent: Wednesday, November 27, 2013 2:54 PM
> To: Bell, Pat
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] TIA1 and granzyme B
>
> Hello to everyone!
>
> Has anyone used
> -Mouse Monoclonal [TIA-1] to TIA1 (ab2712)
> and
> - Mouse anti human granzyme B (Specificity: granzyme B)
> both on FFPE tissue?
>  Could I please get a copy of your protocols?
>
> Thank you so much.
>
> Aldana Vistarop
> Laboratorio Biología Molecular, Servicio de Anatomía Patológica,
> Hospital de Niños Ricardo Gutiérrez.
> Gallo 1330
> C1425EFD
> Ciudad de Buenos Aires
> Argentina
> TE: (5411) 4962-9138.
> FAX: (5411)4962-9138.
>
>
>
>
>
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