[Histonet] TIA1 and granzyme B
CObregon <@t> mhs.net
Wed Nov 27 17:34:29 CST 2013
We are in the completing our validation for both TIA-1 and Granzyme B. Here's what we are using:
TIA (EP243) Rabbit Monoclonal from Cell Marque (Predilute, ready to use)
Protocol for Ventana Ultra is: Extended Retrieval CC1 (64 minutes), antibody incubation 40 minutes, at 37 degrees with amplification.
Granzyme B (Polyclonal) from Cell Marque Catalog#760-4283
Protocol for Ventana Ultra: Mild CC1, at 37 degrees, antibody incubation 16 minutes.
Hope that helps!
Cecilia M. Obregon
Memorial Regional Hospital
3501 Johnson Street
Hollywood, FL 33021
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of avistarop <@t> ffyb.uba.ar [avistarop <@t> ffyb.uba.ar]
Sent: Wednesday, November 27, 2013 2:54 PM
To: Bell, Pat
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] TIA1 and granzyme B
Hello to everyone!
Has anyone used
-Mouse Monoclonal [TIA-1] to TIA1 (ab2712)
- Mouse anti human granzyme B (Specificity: granzyme B)
both on FFPE tissue?
Could I please get a copy of your protocols?
Thank you so much.
Laboratorio Biología Molecular, Servicio de Anatomía Patológica,
Hospital de Niños Ricardo Gutiérrez.
Ciudad de Buenos Aires
TE: (5411) 4962-9138.
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