[Histonet] TIA1 and granzyme B

[Obregon, Cecilia]

We are in the completing our validation for both TIA-1 and Granzyme B. Here's what we are using:

TIA (EP243) Rabbit Monoclonal from Cell Marque (Predilute, ready to use)
Protocol for Ventana Ultra is: Extended Retrieval CC1 (64 minutes), antibody incubation 40 minutes, at 37 degrees with amplification. 

Granzyme B (Polyclonal) from Cell Marque Catalog#760-4283
Protocol for Ventana Ultra: Mild CC1, at 37 degrees, antibody incubation 16 minutes. 

Hope that helps!
Happy Thanksgiving

Cecilia M. Obregon
Memorial Regional Hospital 
3501 Johnson Street
Hollywood, FL 33021
Ph: 954-265-5317

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of avistarop <@t> ffyb.uba.ar [avistarop <@t> ffyb.uba.ar]
Sent: Wednesday, November 27, 2013 2:54 PM
To: Bell, Pat
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] TIA1 and granzyme B

Hello to everyone!

Has anyone used
-Mouse Monoclonal [TIA-1] to TIA1 (ab2712)
and
- Mouse anti human granzyme B (Specificity: granzyme B)
both on FFPE tissue?
 Could I please get a copy of your protocols?

Thank you so much.

Aldana Vistarop
Laboratorio Biología Molecular, Servicio de Anatomía Patológica,
Hospital de Niños Ricardo Gutiérrez.
Gallo 1330
C1425EFD
Ciudad de Buenos Aires
Argentina
TE: (5411) 4962-9138.
FAX: (5411)4962-9138.





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