[Histonet] Rolling of my ribbon ? Another paraffin question.

[Monson, Frederick]

Dear Stella,

Prior to buildings with locked windows, histologists who embedded with paraffin would fabricate, from just paraffin waxes of different hardness to match the climate of their geographic locations.  Thus, 

http://www.microscopy-uk.org.uk/mag/artjan07/yl-para1w.html

you can fabricate your own embedment with/or without the PEG (or whatever hygroscopic agent is added to provide 'stick') to match your needs.  Most large labs will not want to do this, so perhaps adding a dehumidifier to the microtome room to adjust the humidity to the embedment.

An accompanying message will describe one of my favorite mountants that requires an extended prep.

Cheers,

Fred Monson

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of Pennsylvania
Schmucker Science South - Room SSS-024
MailDrop:  Geology-Astronomy
750 South Church Street
West Chester, PA, 19383
610-738-0437
fmonson <@t> wcupa.edu

HomePage:  http://cmirt.wcupa.edu (with link to instrument Scheduler)


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stella Mireles
Sent: Wednesday, November 13, 2013 3:18 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Rolling of my ribbon ? Another paraffin question.

*I am presently using a paraffin designated as an IM product.* *We are a facility that cuts only autopsies and have been experiencing alot of rolling of our sections.* *We did recently switch to this product, because of cost.*

*Question :  Is the paraffin you are using working well on autopsy tissue and producing ribbons right away ? Do you use it for infiltration as well as embedding ?*

*Thank you for your assistant.*


*Stella Walters*
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