[Histonet] Speaking of picrosirius red...

Patsy Ruegg pruegg <@t> ihctech.net
Tue Nov 12 19:31:39 CST 2013

I have done picrosirius red for years and never used  phosphomolybdic acid so it would be hard to say what it's purpose is, mine works fine without it.

> Date: Tue, 12 Nov 2013 19:15:43 -0500
> From: amosbrooks <@t> gmail.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Speaking of picrosirius red...
> Hi,
>      In light of the current discussion about picrosirius red I would like
> to revisit a question that was asked a while back. If it was answered
> publicly, I apologize I probably missed it and didn't see it in the
> archives. Someone asked what is the purpose of the phosphomolybdic acid
> step before the picrosirius red. If anyone has a good answer for this I
> would like to know. While it is mostly curiosity, I think knowing what each
> step in a stain is for helps keep the quality and consistency of stains.
> Thanks,
> Amos
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