[Histonet] Speaking of picrosirius red...

[Caroline Miller]

Same here.......

Caroline Miller
Gladstone Institutes
www.gladstoneinstitutes.org

Tel: 415 7342566
Cell: 415 2187297




> On Nov 12, 2013, at 5:31 PM, Patsy Ruegg <pruegg <@t> ihctech.net> wrote:
> 
> I have done picrosirius red for years and never used  phosphomolybdic acid so it would be hard to say what it's purpose is, mine works fine without it.
> 
>> Date: Tue, 12 Nov 2013 19:15:43 -0500
>> From: amosbrooks <@t> gmail.com
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Speaking of picrosirius red...
>> 
>> Hi,
>>     In light of the current discussion about picrosirius red I would like
>> to revisit a question that was asked a while back. If it was answered
>> publicly, I apologize I probably missed it and didn't see it in the
>> archives. Someone asked what is the purpose of the phosphomolybdic acid
>> step before the picrosirius red. If anyone has a good answer for this I
>> would like to know. While it is mostly curiosity, I think knowing what each
>> step in a stain is for helps keep the quality and consistency of stains.
>> 
>> Thanks,
>> Amos
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