[Histonet] Speaking of picrosirius red...
cmiller <@t> gladstone.ucsf.edu
Tue Nov 12 19:40:00 CST 2013
Tel: 415 7342566
Cell: 415 2187297
> On Nov 12, 2013, at 5:31 PM, Patsy Ruegg <pruegg <@t> ihctech.net> wrote:
> I have done picrosirius red for years and never used phosphomolybdic acid so it would be hard to say what it's purpose is, mine works fine without it.
>> Date: Tue, 12 Nov 2013 19:15:43 -0500
>> From: amosbrooks <@t> gmail.com
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Speaking of picrosirius red...
>> In light of the current discussion about picrosirius red I would like
>> to revisit a question that was asked a while back. If it was answered
>> publicly, I apologize I probably missed it and didn't see it in the
>> archives. Someone asked what is the purpose of the phosphomolybdic acid
>> step before the picrosirius red. If anyone has a good answer for this I
>> would like to know. While it is mostly curiosity, I think knowing what each
>> step in a stain is for helps keep the quality and consistency of stains.
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