AW: [Histonet] Processing thin slices of mouse cerebellum

[Gudrun Lang]

Is the tissue fixed well enough? At least two days?
Processing through graded ethanols with little difference should minimize
shrinkage. Instead of 70-96-100, take 50-60-70-80-96-100. Times shouldn't be
too short. Fatty brain needs more time for reagens-exchange than other small
biopsies.
...smooth protocol.
For embedding one can try to press slightly the tissue with a warm small
metalblock on the ground of the mold 

Gudrun


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
kgrobert <@t> rci.rutgers.edu
Gesendet: Mittwoch, 29. Mai 2013 20:14
An: histonet
Betreff: [Histonet] Processing thin slices of mouse cerebellum

To all,

We have these very thin slices of mouse cerebellum that need to be processed
into paraffin.  The problem is that they need to be kept flat. 
We have tried sponges, but some of the slices are so small that they shrink
(processed with our biopsy program, of course; if you want details, let me
know) and get lost in the holes of the sponges.  We have biopsy cassettes,
but the slices will still tumble and not stay flat. We have processed other
things in lens paper, but I had to scrape the tissue with the paraffin into
the mold.  I have not tried Histogel, though-maybe embed the slices flat in
that first and then process it?  Is there anything else?


Thanks so much!

Kathleen



Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(848) 445-1443
FAX (732) 445-6905

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet