[Histonet] Processing thin slices of mouse cerebellum

[kgrobert <@t> rci.rutgers.edu]

To all,

We have these very thin slices of mouse cerebellum that need to be
processed into paraffin.  The problem is that they need to be kept flat. 
We have tried sponges, but some of the slices are so small that they
shrink (processed with our biopsy program, of course; if you want details,
let me know) and get lost in the holes of the sponges.  We have biopsy
cassettes, but the slices will still tumble and not stay flat. We have
processed other things in lens paper, but I had to scrape the tissue with
the paraffin into the mold.  I have not tried Histogel, though-maybe embed
the slices flat in that first and then process it?  Is there anything
else?


Thanks so much!

Kathleen



Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(848) 445-1443
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