[Histonet] Processing thin slices of mouse cerebellum
ttruscot <@t> vetmed.wsu.edu
Thu May 30 10:46:54 CDT 2013
Hi Kathleen, Several years and a couple employers ago we attached small biopsies (for proper orientation) to small squares of cucumber (which had been dehydrated in several changes of alcohol) with a egg-albumin/glycerol mixture. The bx/cucumber unit was embedded together after processing and cut and stained as a unit with no adverse effects. Tom Truscott
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kgrobert <@t> rci.rutgers.edu
Sent: Wednesday, May 29, 2013 11:14 AM
Subject: [Histonet] Processing thin slices of mouse cerebellum
We have these very thin slices of mouse cerebellum that need to be processed into paraffin. The problem is that they need to be kept flat.
We have tried sponges, but some of the slices are so small that they shrink (processed with our biopsy program, of course; if you want details, let me know) and get lost in the holes of the sponges. We have biopsy cassettes, but the slices will still tumble and not stay flat. We have processed other things in lens paper, but I had to scrape the tissue with the paraffin into the mold. I have not tried Histogel, though-maybe embed the slices flat in that first and then process it? Is there anything else?
Thanks so much!
Principal Lab Technician
Molecular Pathology Facility Core
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
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Piscataway, NJ 08854
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