[Histonet] another Movats question (paraffin)

[koellingr <@t> comcast.net]

Betsy, 
Don't know if applicable to this situation but in the past I have on occasion in other labs noted some stain going off and after checking everything, looked at the tap water. In fact I backtracked through the water utility once to see where the water came from, was delivered and what the utility did to purify. Convinced, although never proven, that tap water was culprit on at least one or two occasions. Utility would say they used "this chemical" to purify but on occasion changed to "that chemical". Although we fall into the habit that tap water is tap water it can change from time to time (source, purification, metals eroding in old pipes, etc). Don't have to be a chemist to drink tap water in one part of US and find it completely different tasting from another part of country. Maybe not likely cause but worth considering. 
Ray 
Research Scientist 
UW Seattle 

----- Original Message -----
From: "Betsy Molinari" <BMolinari <@t> texasheart.org> 
To: Histonet <@t> lists.utsouthwestern.edu 
Sent: Tuesday, May 28, 2013 8:47:19 AM 
Subject: [Histonet] another Movats question (paraffin) 

Hi, 
The Alcian Blue in my Movats is very faint or seems to completely disappear. I have been running Movats for years and this is a new problem. I have checked lot #’s everything is the same. The solution is 1% pH 2.7. The slides are in solution for 20 mins, rinse for 5 mins in tap H2O, then in alkaline alcohol (90 ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed for 10 mins. But after hematoxylin the blue is either gone or very faint. 
Any suggestions? 
Thanks, 
Betsy Molinari HT(ASCP) 
Texas Heart Institute 
Cardiovascular Pathology 
6770 Bertner Ave. 
Houston , TX 77030 
832-355-6524 (lab) 
832-355-6812 (fax) 



<http://www.texasheart.org> 



Betsy Molinari 
Senior Histology Research Technician 
832-355-6524 | BMolinari <@t> texasheart.org<mailto:BMolinari <@t> texasheart.org> | www.texasheart.org<http://www.texasheart.org> 



6770 Bertner Ave., MC 1-283, Houston, TX 77030 



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