[Histonet] another Movats question (paraffin)

Molinari, Betsy BMolinari <@t> texasheart.org
Tue May 28 10:47:19 CDT 2013

The Alcian Blue in my Movats  is very faint or seems to completely disappear. I have been running Movats for years and this is a new problem. I have checked lot #’s everything is the same. The solution is 1% pH 2.7. The slides are in solution for 20 mins, rinse for 5 mins in tap H2O,  then in alkaline alcohol (90 ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed for 10 mins. But after hematoxylin the blue is either gone or very faint.
Any suggestions?
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston , TX 77030
832-355-6524 (lab)
832-355-6812 (fax)


Betsy Molinari
Senior Histology Research Technician
832-355-6524 | BMolinari <@t> texasheart.org<mailto:BMolinari <@t> texasheart.org> | www.texasheart.org<http://www.texasheart.org>

6770 Bertner Ave., MC 1-283, Houston, TX 77030

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