[Histonet] another Movats question (paraffin)

[Molinari, Betsy]

Hi,
The Alcian Blue in my Movats  is very faint or seems to completely disappear. I have been running Movats for years and this is a new problem. I have checked lot #’s everything is the same. The solution is 1% pH 2.7. The slides are in solution for 20 mins, rinse for 5 mins in tap H2O,  then in alkaline alcohol (90 ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed for 10 mins. But after hematoxylin the blue is either gone or very faint.
Any suggestions?
Thanks,
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston , TX 77030
832-355-6524 (lab)
832-355-6812 (fax)



<http://www.texasheart.org>



Betsy Molinari
Senior Histology Research Technician
832-355-6524 | BMolinari <@t> texasheart.org<mailto:BMolinari <@t> texasheart.org> | www.texasheart.org<http://www.texasheart.org>



6770 Bertner Ave., MC 1-283, Houston, TX 77030



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