[Histonet] MDM2 on lipsarcoma IHC

[Gudrun Lang]

Hi all!

We try to improve our MDM2-staining and purchased a new antibody. This one
stains all nuclei in liposarcoma but also in normal surrounding tissue and
lipoma positiv.

On the Protein-Atlas-site MDM2 is described with "Ubiquitous nuclear
expression". On the other hand I read, that well differentiated liposarcoma
are MDM2 negativ, dedifferentiated liposarcoma are positiv.

 

Most likely the point is "overexpression" of MDM2 protein due to
gene-amplification. Maybe we have to "turn down" our protocol to detect only
overexpressed MDM2.

 

Has anyone practical experience with this marker? Can help me? 

 

http://www.proteinatlas.org/ENSG00000135679

 

 

Bye and thanks in advance

Gudrun Lang