[Histonet] MDM2 on lipsarcoma IHC

[Mark Tarango]

Hi Gudrun,

We only do FISH for MDM2 but it is positive for gene amplification in both
well differentiated liposarcomas and dedifferentiated liposarcomas.
Lipomas and other sarcomas should be negative as far as I've seen.  I
haven't worked with the antibody or seen that staining, but I'd guess that
you are right and the protocol needs to be brought down.  That link you
have in your e-mail seems to imply that the antibody stains nearly all
nuclei though.  Maybe the antibody isn't just staining the overexpressed
cells... but all of them? "Ubiquitous nuclear expression" doesn't sound
very specific for the cells that are positive for gene amplification.

Mark


On Mon, May 27, 2013 at 8:37 AM, Gudrun Lang <gu.lang <@t> gmx.at> wrote:

> Hi all!
>
> We try to improve our MDM2-staining and purchased a new antibody. This one
> stains all nuclei in liposarcoma but also in normal surrounding tissue and
> lipoma positiv.
>
> On the Protein-Atlas-site MDM2 is described with "Ubiquitous nuclear
> expression". On the other hand I read, that well differentiated liposarcoma
> are MDM2 negativ, dedifferentiated liposarcoma are positiv.
>
>
>
> Most likely the point is "overexpression" of MDM2 protein due to
> gene-amplification. Maybe we have to "turn down" our protocol to detect
> only
> overexpressed MDM2.
>
>
>
> Has anyone practical experience with this marker? Can help me?
>
>
>
> http://www.proteinatlas.org/ENSG00000135679
>
>
>
>
>
> Bye and thanks in advance
>
> Gudrun Lang
>
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