[Histonet] IHC on non-charged slides
DSiena <@t> statlab.com
Thu May 16 16:00:16 CDT 2013
There is a procedure where you can transfer the sections to a charges slide with a product called mount quick. You can check with newcomer supply they sell it. I know of several labs that have used it on these occasions and it works well.
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On May 16, 2013, at 4:50 PM, "Dessoye, Michael J" <mjdessoye <@t> commonwealthhealth.net> wrote:
> I'm hoping Histonet can come through for me! I have an unusual case that I need to run IHC on. Antibody is Pan Keratin Cocktail from Cell Marque with recommended protocol on a Benchmark Ultra with i-view detection. Only problem is, I only have two slides and they are not charged. The tissues have been air-dried but not baked. I cannot obtain more slides.
> I'm wondering if there's any kind of pre-treatment I could try to try to help the tissue stay on. We have run slides like this in the past with mixed results. Most of the time, the tissue washes off.
> Any tips or tricks that might help out in this situation?
> Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye <@t> commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526
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