[Histonet] IHC on non-charged slides
marktarango <@t> gmail.com
Thu May 16 16:12:06 CDT 2013
Yes, if the stain can wait get some mount quick from Newcomer. There is a
good chance the tissue will fall off if you proceed with the tissue on
regular slides. I would wait until I get the mount quick.
Everyone else reading this should order a tube now for emergencies. You
will find yourself in this position at some point and if you have the stuff
on hand, it's no problem.
I just used it today to transfer cells from a cytocentrifuge slide to a
charged slide for FISH. It saved the patient a re-biopsy.
On Thu, May 16, 2013 at 2:00 PM, Debra Siena <DSiena <@t> statlab.com> wrote:
> There is a procedure where you can transfer the sections to a charges
> slide with a product called mount quick. You can check with newcomer supply
> they sell it. I know of several labs that have used it on these occasions
> and it works well.
> Sent from my iPhone
> On May 16, 2013, at 4:50 PM, "Dessoye, Michael J" <
> mjdessoye <@t> commonwealthhealth.net> wrote:
> > I'm hoping Histonet can come through for me! I have an unusual case
> that I need to run IHC on. Antibody is Pan Keratin Cocktail from Cell
> Marque with recommended protocol on a Benchmark Ultra with i-view
> detection. Only problem is, I only have two slides and they are not
> charged. The tissues have been air-dried but not baked. I cannot obtain
> more slides.
> > I'm wondering if there's any kind of pre-treatment I could try to try to
> help the tissue stay on. We have run slides like this in the past with
> mixed results. Most of the time, the tissue washes off.
> > Any tips or tricks that might help out in this situation?
> > Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
> Hospital | An Affiliate of Commonwealth Health |
> mjdessoye <@t> commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA
> 18764 | Tel: 570-552-1432 | Fax: 570-552-1526
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