[Histonet] IHC on non-charged slides

Rene J Buesa rjbuesa <@t> yahoo.com
Thu May 16 15:53:55 CDT 2013

After the sections are on the slides there is little (is anything) you can do to make them more resistant to the IHC protocol.
My suggestions is to perform the procedure manually and instead of HIER use enzymatic retrieval that is less "traumatic" to the sections.
René J.

From: "Dessoye, Michael J" <mjdessoye <@t> commonwealthhealth.net>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Thursday, May 16, 2013 4:49 PM
Subject: [Histonet] IHC on non-charged slides

I'm hoping Histonet can come through for me!  I have an unusual case that I need to run IHC on.  Antibody is Pan Keratin Cocktail from Cell Marque with recommended protocol on a Benchmark Ultra with i-view detection.  Only problem is, I only have two slides and they are not charged.  The tissues have been air-dried but not baked.  I cannot obtain more slides.

I'm wondering if there's any kind of pre-treatment I could try to try to help the tissue stay on.  We have run slides like this in the past with mixed results.  Most of the time, the tissue washes off.

Any tips or tricks that might help out in this situation?

Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye <@t> commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 
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