[Histonet] RE: ventana counterstaining

[Kimmie Rabe]

Hematoxylin and bluing on the ventana.  But it is run through alcohols to xylene to coverslip.  Possibly??  Although the histotechs say it comes off the ventana with the pink tint. 

Kim Rabe

-----Original Message-----
From: Lori Gemeinhardt [mailto:lorigemeinhardt <@t> mac.com] 
Sent: Thursday, March 21, 2013 3:25 PM
To: Sebree Linda A
Cc: Kimmie Rabe; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: ventana counterstaining

Hi Kimmie-
Are you running your slides down in the routine H&E set up, ie are you using eosin 'contaminated' alcohols. Maybe you are lingering too long, and it is picking up some Eosin. Maybe??

Thanks-
Lori

On Mar 21, 2013, at 12:41 PM, Sebree Linda A <LSebree <@t> uwhealth.org> wrote:

> Kimmie,
> 
> We use VMS' Hematoxylin II with no changes from how it has always stained.
> 
> 
> 
> Linda A. Sebree
> 
> University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 
> Highland Ave.
> Madison, WI 53792
> 
> (608)265-6596
> FAX: (608)262-7174
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu 
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kimmie 
> Rabe
> Sent: Thursday, March 21, 2013 11:28 AM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] ventana counterstaining
> 
> Has anyone else had problems with the counterstain on the ventana being pink instead of that lovely pale blue color?  Ventana says it's because we use Sta-On, but we don't always use Sta-On and the pink vs. blue staining doesn't correlate with whether Sta-On has been used or not.  Don't get me wrong, I have nothing against pink.  But if there is some problem with the hematoxylin we purchase from ventana or the dispensing or mixing thereof--we would like to know.
> 
> Thanks!
> 
> Kimmie E. Rabe, MD
> North Central Pathology, PA
> 3701 12th Street North, Suite 201
> St. Cloud, MN 56303
> Phone:  320-253-6554
> Fax:  320-253-1218
> kim <@t> ncpath.com<mailto:kim <@t> ncpath.com>
> 
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