[Histonet] Sirius Red Stain Troubleshooting

[Lillian Chau]

Hi All-


We have previously used Llewellyn sirius red protocol from Stainsfile with no issues to stain paneth cells.  

Recently, the sections are looking more like an H and E stain or completely red rather than the expected light blue (nuclei) and red (paneth) stain that we are accustomed to.

The powder is from Sigma (its 2 years old) and we make a fresh solution for use each time.  

Hopefully you can provide some insight regarding this recent problem we are having.

Thanks,
Lillian