[Histonet] Mouse bone blocks/sections, bone marrow smears

[Sean McBride]

Hi Jian,

I specialize in orthopedic histology, and I also live in Pittsburgh.  I would be glad to discuss the details of your project and see if I can be of any assistance to you.  Please contact me at your convenience.


Best regards,


~Sean McBride


Scientific Specialist
Bone Tissue Engineering Center
Carnegie Mellon Research Institute
700 Technology Drive
Pittsburgh, PA 15219-3124

571-989-BONE (m)
412-268-8275 (fax)
seanmcbride007 <@t> me.com



On Mar 21, 2013, at 7:26 PM, Yu, Jian wrote:

> Dear all,
> 
> We like to make paraffin embedded bone of mouse tibia and cut longitudinal sections for H&E sections. 
> 
> Does anyone have a easy-to-follow protocol to prepare (fix and decal) the bone before processing? We routinely fix soft tissue (ie. mouse GI tract  and liver) and send off to our pathology core for processing and embedding. 
> 
> Any tips on cutting the blocks is helpful too. These sections appear brittle.
> 
> We are also interested in a practical protocol for making mouse BM smears and staining to determine cellularity changes after radiation.
> 
> We just started working with the bone.  Any advice will be very helpful and greatly appreciated.
> 
> Thanks, Jian
> *******************************************************************
> Jian Yu, Ph.D.
> University of Pittsburgh Cancer Institute
> Email: yuj2 <@t> upmc.edu
> *******************************************************************
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>