[Histonet] EMA,clone 23 dog

[Jan Shivers]

I have never had success in getting an EMA to cross-react with dog tissue
(that particular clone epitope not conserved across species).  If he has
any success, I would be grateful to find out what antibody company and
clone he uses.

Best regards,
Jan Shivers
Senior Scientist
University of Minnesota
Veterinary Diagnostic Lab

On Fri, Mar 15, 2013 at 3:55 AM, Gerard Spoelstra <
G.Spoelstra <@t> murdoch.edu.au> wrote:

> Hi everyone,
>
>
>
> My colleague has been trying without success to run the monoclonal EMA ,
> clone23 on a dog. He has tried ph9 tris buffer,ph 6 citrate/edta buffer
> and proteinase k for an hour using a  1/200 dilution and Dako envision.
>
> Any suggestions.
>
>
>
> Gerard Spoelstra
>
> Murdoch University
>
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