[Histonet] problem with DAB staining

[Rob Geske]

Did you use a serum block ahead of your primary antibody ?  If yes, what species was it derived from?

In what species was your primary antibody generated ?



--- On Thu, 3/7/13, Nicole Cosenza <ncosenza <@t> siumed.edu> wrote:

From: Nicole Cosenza <ncosenza <@t> siumed.edu>
Subject: [Histonet] problem with DAB staining
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, March 7, 2013, 11:50 AM

Hello all:

I recently did IHC on paraffin embedded slides via the DAB method. Upon addition of the DAB, the entire section turned brown. I know this is not true staining, as I don't expect a high yield of my protein of interest. Any suggestions as to why this happened and what to do about it?

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet