[Histonet] problem with DAB staining

tahseen tahseen <@t> brain.net.pk
Thu Mar 7 12:20:33 CST 2013



Section should not dry in any stapes.

Tahseen

On 2013-03-07 22:50, Nicole Cosenza wrote:
> Hello all:
>
> I recently did IHC on paraffin embedded slides via the DAB method.
> Upon addition of the DAB, the entire section turned brown. I know 
> this
> is not true staining, as I don't expect a high yield of my protein of
> interest. Any suggestions as to why this happened and what to do 
> about
> it?
>
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