[Histonet] problem with DAB staining

[Rena Fail]

The most likey reasons are the tissue dried out at some point or the slide
was not adequately  rinsed between steps
Rena Fail

On Thu, Mar 7, 2013 at 1:39 PM, Rob Geske <rgeske_2000 <@t> yahoo.com> wrote:

> Did you use a serum block ahead of your primary antibody ?  If yes, what
> species was it derived from?
>
> In what species was your primary antibody generated ?
>
>
>
> --- On Thu, 3/7/13, Nicole Cosenza <ncosenza <@t> siumed.edu> wrote:
>
> From: Nicole Cosenza <ncosenza <@t> siumed.edu>
> Subject: [Histonet] problem with DAB staining
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Thursday, March 7, 2013, 11:50 AM
>
> Hello all:
>
> I recently did IHC on paraffin embedded slides via the DAB method. Upon
> addition of the DAB, the entire section turned brown. I know this is not
> true staining, as I don't expect a high yield of my protein of interest.
> Any suggestions as to why this happened and what to do about it?
>
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