[Histonet] problem with DAB staining

[Robert Schoonhoven]

We would need to see the entire set of steps as there may be a variety of causes.


Robert Schoonhoven, HT/HTL (ASCP)


________________________________
From: Nicole Cosenza <ncosenza <@t> siumed.edu>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Thursday, March 7, 2013 12:50 PM
Subject: [Histonet] problem with DAB staining

Hello all:

I recently did IHC on paraffin embedded slides via the DAB method. Upon addition of the DAB, the entire section turned brown. I know this is not true staining, as I don't expect a high yield of my protein of interest. Any suggestions as to why this happened and what to do about it?

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