[Histonet] making 10 % EDTA

[Jim Burchette]

Don't forget EDTA doesn't fully go into solution until the pH approaches
8.0. You will need to prepare a sodium hydroxide solution for pH
adjustment.


On Wed, Jun 26, 2013 at 3:46 PM, Goins, Tresa <TGoins <@t> mt.gov> wrote:

> The molecular weight of the EDTA will vary depending on chemical makeup -
> regardless, the solution you have is probably less than 10% already.  EDTA
> tri-sodium salt with MW 358 at 0.25 M is 89.5 g per liter or 8.95 g per 100
> ml or 8.95%.
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle
> Sent: Wednesday, June 26, 2013 12:37 PM
> To: histonet
> Subject: [Histonet] making 10 % EDTA
>
> Hi Histonetters,
>
> I have purchased a liquid EDTA solution that is .25M. I need to make 10%
> EDTA.  Do I just dilute it 1/10 with DI water?
>
>
> Thanks
>
>
> Kimberly C. Tuttle  HT (ASCP)
> Pathology Biorepository and Research Core University of Maryland Room
> NBW58, UMMC
> 22 S. Greene St
> Baltimore, MD 21201
> Pager#5029
> (410) 328-5558
> (410) 328-5508 fax
> https://cf.umaryland.edu/freezer/promo_pbr.cfm
>
>
>
>
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-- 
Jim Burchette
"Fly Fishing Bum"  *<'(((><*