[Histonet] making 10 % EDTA

Goins, Tresa TGoins <@t> mt.gov
Wed Jun 26 14:46:49 CDT 2013

The molecular weight of the EDTA will vary depending on chemical makeup - regardless, the solution you have is probably less than 10% already.  EDTA tri-sodium salt with MW 358 at 0.25 M is 89.5 g per liter or 8.95 g per 100 ml or 8.95%.  

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle
Sent: Wednesday, June 26, 2013 12:37 PM
To: histonet
Subject: [Histonet] making 10 % EDTA

Hi Histonetters,
I have purchased a liquid EDTA solution that is .25M. I need to make 10% EDTA.  Do I just dilute it 1/10 with DI water?
Kimberly C. Tuttle  HT (ASCP)
Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5558
(410) 328-5508 fax


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