[Histonet] Fatty Fixation
Lee & Peggy Wenk
lpwenk <@t> sbcglobal.net
Fri Jun 21 15:19:01 CDT 2013
What exactly is wrong with the fatty specimens?
If the nuclei look smudgy, with no nuclear detail, then it has not been
fixed long enough.
If the fat is still in the tissue and you cannot section it on a microtome,
then the tissue has not had enough time during processing, especially length
of time in xylene and paraffin. So it would be a processing problem, not a
fixation problem. And possibly a grossing problem, if the fatty tissue is
grossed to thick for the length of time on the processor.
Peggy A. Wenk, HTL(ASCP)SLS
Royal Oak, MI 48073
Opinions expressed do not reflect on the hospital.
From: White, Lisa M.
Sent: Thursday, June 20, 2013 2:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fatty Fixation
Does anyone have a method they will share to fix fatty specimens? Does
anyone utilize a stir plate? Any help greatly appreciated.
We currently use Alcoholic Formalin but the results are not reliable.
Lisa White HT(ASCP)
James H. Quillen VAMC
Corner of Veterans Way and Lamont
VAMC Warehouse BLDG. 205
PO Box 4000
Mountain Home, TN 37684
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