[Histonet] Fatty Fixation

[Massimo]

You can fix in Flemming's Liquid or, better in an aqueous solution
of Osmic Acid at 1% for 24 hours.
The Osmic acid has also the property to blacken fats.

Kind Regards,
Massimo Tosi


________________________________
 Da: Rene J Buesa <rjbuesa <@t> yahoo.com>
A: "White, Lisa M." <Lisa.White3 <@t> va.gov>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Inviato: Giovedì 20 Giugno 2013 22:38
Oggetto: Re: [Histonet] Fatty Fixation
 

The answer is time, time, time (>36h)
René J.

From: "White, Lisa M." <Lisa.White3 <@t> va.gov>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Thursday, June 20, 2013 2:23 PM
Subject: [Histonet] Fatty Fixation


Does anyone have a method they will share to fix fatty specimens?  Does
anyone utilize a stir plate?  Any help greatly appreciated.  

We currently use Alcoholic Formalin but the results are not reliable.





Lisa White HT(ASCP)

Supervisory HT

James H. Quillen VAMC

Corner of Veterans Way and Lamont

VAMC Warehouse BLDG. 205

PO Box 4000
PLMS 113

Mountain Home, TN 37684

423-979-3567

423-979-3401 fax



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet