[Histonet] Fatty Fixation

Sarah Dysart sdysart <@t> mirnarx.com
Mon Jun 24 08:24:42 CDT 2013

Try formalin-aceto-alcohol (F-A-A)...it works pretty well =)

Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk
Sent: Friday, June 21, 2013 3:19 PM
To: White, Lisa M.; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Fatty Fixation

What exactly is wrong with the fatty specimens?

If the nuclei look smudgy, with no nuclear detail, then it has not been 
fixed long enough.

If the fat is still in the tissue and you cannot section it on a microtome, 
then the tissue has not had enough time during processing, especially length 
of time in xylene and paraffin. So it would be a processing problem, not a 
fixation problem. And possibly a grossing problem, if the fatty tissue is 
grossed to thick for the length of time on the processor.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

Opinions expressed do not reflect on the hospital.

-----Original Message----- 
From: White, Lisa M.
Sent: Thursday, June 20, 2013 2:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fatty Fixation

Does anyone have a method they will share to fix fatty specimens?  Does
anyone utilize a stir plate?  Any help greatly appreciated.

We currently use Alcoholic Formalin but the results are not reliable.

Lisa White HT(ASCP)

Supervisory HT

James H. Quillen VAMC

Corner of Veterans Way and Lamont

VAMC Warehouse BLDG. 205

PO Box 4000
PLMS 113

Mountain Home, TN 37684


423-979-3401 fax

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