[Histonet] Karnovsky's

[Molinari, Betsy]

Rene,
Thank you for your response. It is a whole heart. We have documented the arrival condition and took pictures. I took a small piece and processed it last night and will be cutting it in a few minutes. I am pretty sure the results will be sub-optimal.  This will be for documentation.
Thanks again,
Betsy




<http://www.texasheart.org>



Betsy Molinari
Senior Histology Research Technician
832-355-6524 | BMolinari <@t> texasheart.org<mailto:BMolinari <@t> texasheart.org> | www.texasheart.org<http://www.texasheart.org>



6770 Bertner Ave., MC 1-283, Houston, TX 77030



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From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
Sent: Wednesday, June 19, 2013 1:21 PM
To: Molinari, Betsy; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Karnovsky's

Are you referring to a whole heart? If that is the case I think the fixation of the tissue will be very poor because Karnovsky's penetrate very slowly.
I do not think it will be worth the effort and materials to process this tissue.
René J.

From: "Molinari, Betsy" <BMolinari <@t> texasheart.org<mailto:BMolinari <@t> texasheart.org>>
To: "Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>" <Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>>
Sent: Wednesday, June 19, 2013 1:32 PM
Subject: [Histonet] Karnovsky's

Hi,
I received a heart that was fixed in 2% Karnovsky’s. I was told it was initially kept under refrigeration but then stored at RT. I do not know how long  the heart has been stored at RT. By the look of the tissue I would say quite a while. Is this tissue compromised in any way? Should I remove it from the Karnovsky’s and store in in something else? This heart is to undergo paraffin processing.
Thanks for any input.
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
MC1-283
Houston,TX 77030
832-355-6524 (lab)
832-355-6812 (fax)



<http://www.texasheart.org>



Betsy Molinari
Senior Histology Research Technician
832-355-6524 | BMolinari <@t> texasheart.org<mailto:BMolinari <@t> texasheart.org><mailto:BMolinari <@t> texasheart.org<mailto:BMolinari <@t> texasheart.org>> | www.texasheart.org<http://www.texasheart.org/>



6770 Bertner Ave., MC 1-283, Houston, TX 77030



[Texas Heart Institute]<https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html>[THI News] [THI on Facebook] <http://www.facebook.com/Texas.Heart.Institute>  [THI on Flicker] <http://www.flickr.com/photos/texasheart/sets/>  [THI on Google] <https://plus.google.com/u/0/118043615690351997044/posts>  [THI on Pinterest] <http://pinterest.com/texasheartinst/>  [THI on Twitter] <http://twitter.com/Texas_Heart>  [THI on You Tube] <http://www.youtube.com/TexasHeartInstitute>
Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system.

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