[Histonet] Karnovsky's

Rene J Buesa rjbuesa <@t> yahoo.com
Wed Jun 19 13:20:41 CDT 2013

Are you referring to a whole heart? If that is the case I think the fixation of the tissue will be very poor because Karnovsky's penetrate very slowly.
I do not think it will be worth the effort and materials to process this tissue.
René J.

From: "Molinari, Betsy" <BMolinari <@t> texasheart.org>
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu> 
Sent: Wednesday, June 19, 2013 1:32 PM
Subject: [Histonet] Karnovsky's

I received a heart that was fixed in 2% Karnovsky’s. I was told it was initially kept under refrigeration but then stored at RT. I do not know how long  the heart has been stored at RT. By the look of the tissue I would say quite a while. Is this tissue compromised in any way? Should I remove it from the Karnovsky’s and store in in something else? This heart is to undergo paraffin processing.
Thanks for any input.
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston,TX 77030
832-355-6524 (lab)
832-355-6812 (fax)


Betsy Molinari
Senior Histology Research Technician
832-355-6524 | BMolinari <@t> texasheart.org<mailto:BMolinari <@t> texasheart.org> | www.texasheart.org<http://www.texasheart.org/>

6770 Bertner Ave., MC 1-283, Houston, TX 77030

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