[Histonet] Picro Sirius Red Stain

[Laura Avogaro]

Dear John,
I usually perform Picrosirius Red stain in cardiac tisse sections.
I use phosphomolibic acid treatment in order to eliminate the cytoplasmic
staining improving collagen/myoplasm contrast.

Have a look at this paper:
Picrosirius red staining of cardiac muscle following phosphomolybdic acid
treatment
Dolber PC, Spach MS
Stain Technol. 1987 Jan;62(1):23-6.

Good luck!

Laura


> Hi All,
>
> Hope all are having a great Monday!!!!
>
> I am trying to do a Picro Sirius Red stain and have looked at many
> protocols that seem to not use a particular reagent that is in a
> manufacturers kit. I was hoping if someone could enlighten me as to why
> this reagent would be used and if someone is using it at what strength is
> the PhosoMolybdic Acid concentration.
>
> Your help will be greatly appreciated!!!!
>
> Kind Regards!
>
> John J Shelley
> Research Specialist, Histology Core Facility
>
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Laura Avogaro
University of Trento
Via delle Regole, 101 38123 Mattarello (TN) – Italy
Tel:  +39 0461 283425