[Histonet] Processing Guinea Pig

Heather Marlatt hmarlatt26 <@t> gmail.com
Mon Jun 17 09:33:21 CDT 2013

Hello Histonet! I'm a long time reader first time poster. Does anyone have
experience processing guinea pig tissues? I have been processing kidney and
heart but it is consistently coming out mushy in the middle. The mouse
tissue comes out fine even when processed on the same run. I had the tissue
grossed in thinner (2.5mm) thinking that perhaps it was too thick but it
didn't seem to help. Also, it has been fixed in 10%NBF for several days.

I was just wondering if anyone else had similar problems with guinea pig?

I appreciate in advance any advice or tips.

Here is the protocol:

Formalin 1hr
70% etOH 1hr
95% 1hr
100% 30min
100% 1hr
100% 1hr
Clearify 1hr
Clearify  1hr
Clearify 1hr
Paraffin 1hr
paraffin 1hr

All under pressure and heat only on the paraffin.


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