[Histonet] Processing Guinea Pig
hmarlatt26 <@t> gmail.com
Mon Jun 17 09:33:21 CDT 2013
Hello Histonet! I'm a long time reader first time poster. Does anyone have
experience processing guinea pig tissues? I have been processing kidney and
heart but it is consistently coming out mushy in the middle. The mouse
tissue comes out fine even when processed on the same run. I had the tissue
grossed in thinner (2.5mm) thinking that perhaps it was too thick but it
didn't seem to help. Also, it has been fixed in 10%NBF for several days.
I was just wondering if anyone else had similar problems with guinea pig?
I appreciate in advance any advice or tips.
Here is the protocol:
70% etOH 1hr
All under pressure and heat only on the paraffin.
More information about the Histonet