[Histonet] Picro Sirius Red Stain

Grantham, Andrea L - (algranth) algranth <@t> email.arizona.edu
Mon Jun 17 10:28:30 CDT 2013

I do a Picro Sirius Red stain for collagen (got the protocol from Gayle Callis) and don't use Phosphomolybdic acid.

My protocol is simple:
one hour in PSR
two rinses in acidified water
you can counterstain but my clients don't want a counterstain so I dehydrate, clear and coverslip.
Boom, I'm done.

The PSR stain is just Sirius Red F3B 0.5 gms and 500 ml of Sat. Picric Acid. Do not use a dye that is not CI 35780.
The acidified water is 5ml Glacial Acetic Acid to 1L of DH2O

Under polarized light the PSR stain is gorgeous! I love looking at the orange, yellow, pinks and green colors. Good thing because I do this stain all the time.

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097

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