[Histonet] RE: Cutting paraffin sections...on a cryostat?

Connolly, Brett M brett_connolly <@t> merck.com
Fri Jun 14 15:21:52 CDT 2013

Sure it could be done - low throughput and very awkward though.  So, no other lab at the university has a microtome that they would let a fellow researcher get some time on ??? 

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin
Sent: Friday, June 14, 2013 4:09 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Cutting paraffin sections...on a cryostat?

Hi, all.  A bit of an odd question: a colleague knows of someone wanting to cut paraffin sections who has a cryostat, but no microtome. Since a cryostat's basically a microtome in a freezer chamber, I thought that it may be awkward, but theoretically doable once it was brought to room temp and dried out thoroughly. However, I wondered if lubricants formulated for the cold might become too thin for use at room temp, possibly causing damage to moving parts.  Any thoughts?

Kevin Johnson
University of Miami
Diabetes Research Institute
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