[Histonet] Cutting paraffin sections...on a cryostat?
Rene J Buesa
rjbuesa <@t> yahoo.com
Fri Jun 14 15:51:34 CDT 2013
It is theoretically possible although very "odd" to say the last. Never mind the lubricants. The only thing is that the HT may end with a terrible back pain.
From: "Johnson, Kevin" <KJohnson <@t> med.miami.edu>
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, June 14, 2013 4:09 PM
Subject: [Histonet] Cutting paraffin sections...on a cryostat?
Hi, all. A bit of an odd question: a colleague knows of someone wanting to cut paraffin sections who has a cryostat, but no microtome. Since a cryostat's basically a microtome in a freezer chamber, I thought that it may be awkward, but theoretically doable once it was brought to room temp and dried out thoroughly. However, I wondered if lubricants formulated for the cold might become too thin for use at room temp, possibly causing damage to moving parts. Any thoughts?
University of Miami
Diabetes Research Institute
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