[Histonet] Cutting paraffin sections...on a cryostat?

Rene J Buesa rjbuesa <@t> yahoo.com
Fri Jun 14 15:51:34 CDT 2013

It is theoretically possible although very "odd" to say the last. Never mind the lubricants. The only thing is that the HT may end with a terrible back pain.
René J.

From: "Johnson, Kevin" <KJohnson <@t> med.miami.edu>
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Friday, June 14, 2013 4:09 PM
Subject: [Histonet] Cutting paraffin sections...on a cryostat?

Hi, all.  A bit of an odd question: a colleague knows of someone wanting to cut paraffin sections who has a cryostat, but no microtome. Since a cryostat's basically a microtome in a freezer chamber, I thought that it may be awkward, but theoretically doable once it was brought to room temp and dried out thoroughly. However, I wondered if lubricants formulated for the cold might become too thin for use at room temp, possibly causing damage to moving parts.  Any thoughts?

Kevin Johnson
University of Miami
Diabetes Research Institute
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