[Histonet] RE: Cutting paraffin sections...on a cryostat?

Sarah Dysart sdysart <@t> mirnarx.com
Fri Jun 14 15:14:59 CDT 2013

Technically you are right...it's just a really cold tome.  I guess you could cut sections with it...it would be super awkward though you're right.  I wouldn't use normal tome oil on it because if you did decide to turn it back on, the normal oil will freeze up on you.  Just continue to use the cryostat oil and you should be fine.
Tell your colleague good luck!!

Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin
Sent: Friday, June 14, 2013 3:09 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Cutting paraffin sections...on a cryostat?

Hi, all.  A bit of an odd question: a colleague knows of someone wanting to cut paraffin sections who has a cryostat, but no microtome. Since a cryostat's basically a microtome in a freezer chamber, I thought that it may be awkward, but theoretically doable once it was brought to room temp and dried out thoroughly. However, I wondered if lubricants formulated for the cold might become too thin for use at room temp, possibly causing damage to moving parts.  Any thoughts?

Kevin Johnson
University of Miami
Diabetes Research Institute
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