[Histonet] Background on Jones Stain

Jay Lundgren jaylundgren <@t> gmail.com
Wed Jul 31 12:41:59 CDT 2013

I'd guess it's silver residue.  For some reason, non-specific silver is
sticking to the new slides.  Why don't you just buy a case of your old
slides to keep around for the Jones stain?


 Jay A. Lundgren, M.S., HTL (ASCP)

On Tue, Jul 30, 2013 at 5:00 PM, Vickroy, Jim <Vickroy.Jim <@t> mhsil.com> wrote:

> Recently we switched to a different hydrophilic slide for our special
> stains and IHC stains.   Everything is working great except for one
> stain......the Jones Silver stain.  There is a brown stain all over the
> slide except where the tissue is.   It appears that the Jones has worked.
>  Everyone of our other silver stains work fine with this new slide.   We
> use Ventana stainers and when we use a different hydrophilic slide or even
> some hydrophobic slides the Jones does not have this background stain all
> over the slide.   Any ideas on what is happening or has anyone else
> experienced this problem?
> James Vickroy BS, HT(ASCP)
> Surgical  and Autopsy Pathology Technical Supervisor
> Memorial Medical Center
> 217-788-4046
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