[Histonet] nanodrop of DNA preps from paraffin sections

Lewis, Patrick patrick.lewis <@t> seattlechildrens.org
Tue Jul 30 18:13:27 CDT 2013

Hi Everyone,

Just out of curiosity, what sort of nanodrop values do you typically get with DNA extractions from paraffin sections.

I usually get around 100 ng/uL, depending on tissue size, and the number of churls I take. Around 80% are 100 ng to 200 ng/uL.

Rarely, I get as high as 1000 ng/uL, 0-5% of the samples I take.
Occasionally I get 10-40 ng/uL 5-15% of the samples I take.

I primarily extract from tonsil, but I have done several other tissue types.


I also primarily use QIAgen Mini kits.

CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.

More information about the Histonet mailing list