[Histonet] nanodrop of DNA preps from paraffin sections
patrick.lewis <@t> seattlechildrens.org
Tue Jul 30 18:13:27 CDT 2013
Just out of curiosity, what sort of nanodrop values do you typically get with DNA extractions from paraffin sections.
I usually get around 100 ng/uL, depending on tissue size, and the number of churls I take. Around 80% are 100 ng to 200 ng/uL.
Rarely, I get as high as 1000 ng/uL, 0-5% of the samples I take.
Occasionally I get 10-40 ng/uL 5-15% of the samples I take.
I primarily extract from tonsil, but I have done several other tissue types.
I also primarily use QIAgen Mini kits.
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