[Histonet] Background on Jones Stain

Vickroy, Jim Vickroy.Jim <@t> mhsil.com
Tue Jul 30 17:00:25 CDT 2013

Recently we switched to a different hydrophilic slide for our special stains and IHC stains.   Everything is working great except for one stain......the Jones Silver stain.  There is a brown stain all over the slide except where the tissue is.   It appears that the Jones has worked.  Everyone of our other silver stains work fine with this new slide.   We use Ventana stainers and when we use a different hydrophilic slide or even some hydrophobic slides the Jones does not have this background stain all over the slide.   Any ideas on what is happening or has anyone else experienced this problem?

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center

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