AW: [Histonet] nanodrop of DNA preps from paraffin sections

[Gudrun Lang]

It depends on...
We isolate DNA from very small biopsies -- 10-40 ng/µl (10 slices, measured
with Qubit; Nanodrop is usuall about 2-3 fold more).
>From surgical tissue -- 50-150 ng/µl (1 cm2, 3-4 slices)

We use the Qiacube with filter columns for isolation, so too much DNA would
be sticking in the silicate-membrane.

What is the question behind your question?
Gudrun

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Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Lewis,
Patrick
Gesendet: Mittwoch, 31. Juli 2013 01:13
An: 'Histonet <@t> lists.utsouthwestern.edu'
Betreff: [Histonet] nanodrop of DNA preps from paraffin sections

Hi Everyone,

Just out of curiosity, what sort of nanodrop values do you typically get
with DNA extractions from paraffin sections.

I usually get around 100 ng/uL, depending on tissue size, and the number of
churls I take. Around 80% are 100 ng to 200 ng/uL.

Rarely, I get as high as 1000 ng/uL, 0-5% of the samples I take.
Occasionally I get 10-40 ng/uL 5-15% of the samples I take.

I primarily extract from tonsil, but I have done several other tissue types.

Patrick.

I also primarily use QIAgen Mini kits.


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