AW: [Histonet] nanodrop of DNA preps from paraffin sections

Gudrun Lang gu.lang <@t>
Wed Jul 31 09:13:29 CDT 2013

It depends on...
We isolate DNA from very small biopsies -- 10-40 ng/µl (10 slices, measured
with Qubit; Nanodrop is usuall about 2-3 fold more).
>From surgical tissue -- 50-150 ng/µl (1 cm2, 3-4 slices)

We use the Qiacube with filter columns for isolation, so too much DNA would
be sticking in the silicate-membrane.

What is the question behind your question?

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von Lewis,
Gesendet: Mittwoch, 31. Juli 2013 01:13
An: 'Histonet <@t>'
Betreff: [Histonet] nanodrop of DNA preps from paraffin sections

Hi Everyone,

Just out of curiosity, what sort of nanodrop values do you typically get
with DNA extractions from paraffin sections.

I usually get around 100 ng/uL, depending on tissue size, and the number of
churls I take. Around 80% are 100 ng to 200 ng/uL.

Rarely, I get as high as 1000 ng/uL, 0-5% of the samples I take.
Occasionally I get 10-40 ng/uL 5-15% of the samples I take.

I primarily extract from tonsil, but I have done several other tissue types.


I also primarily use QIAgen Mini kits.

CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is
for the sole use of the intended recipient(s) and may contain confidential
and privileged information protected by law. Any unauthorized review, use,
disclosure or distribution is prohibited. If you are not the intended
recipient, please contact the sender by reply e-mail and destroy all copies
of the original message.

Histonet mailing list
Histonet <@t>

More information about the Histonet mailing list