[Histonet] Section cells on a plastic trans well membrane

Erickson, Jamie E jamie.erickson <@t> abbvie.com
Tue Jul 9 15:52:32 CDT 2013

Hi All,
                  Has anyone tried to section cells cut from a trans well dish? We have cells that are cultured and attach to a trans well membrane and our pathologist wants to look at them on the membrane but the membrane is a thin plastic film...see the problem.
We fixed the cells in the trans well for 5 minutes then  cut the trans well out of the well and bisected it and processed overnight we use paraffin  type #1 paraffin. The next morning we embedded it with type #9 paraffin.  The samples cut but  do not stay on the slide after xylene. I think the plastic does not adhere to the slide. Would a adhesive slide work to keep the thin plastic stuck to the slide??

My pathologist does not want to change to a collagen membrane  so he wants me to find another (harder paraffin) to use, I don't think that will help.
Has anyone does similar processing/embedding?
I'll take any help I can get..


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