Re: [Histonet] periodic acid/h&e

[ewj <@t> pigsqq.org]

I have an ancient copy of Humason and she had methods 2 to 5 minutes for PAS etc with Periodic Acid so I tried 2 min today.  Will try more time and stronger solutions and will also get around to doing some staining with PAS that I wanted to do to look at goblet cells and compare them to the vacuolated cells produced by porcine epidemic diarrhea...


>  -------Original Message-------
>  From: Akemi Allison <akemiat3377 <@t> yahoo.com>
>  To: ewj <@t> pigsqq.org <ewj <@t> pigsqq.org>, histonet <@t> lists.utsouthwestern.edu <histonet <@t> lists.utsouthwestern.edu>
>  Subject: Re: [Histonet] periodic acid/h&e
>  Sent: Jul 05 '13 22:38
>  
>  I forgot to mention the timing.  You may try 5 minutes or even 10 minutes before staining in hematoxylin. It will give you even better results. I used 5 minutes in 1 % periodic acid.
>   
>  Akemi Allison BS, HT(ASCP)HTL
>  Director
>  Phoenix Lab Consulting
>  E-Mail: akemiat3377 <@t> yahoo.com
>  
>  
>  
>  
>  ________________________________
>  From: "ewj <@t> pigsqq.org" <ewj <@t> pigsqq.org>
>  To: histonet <@t> lists.utsouthwestern.edu; """ <histonet <@t> lists.utsouthwestern.edu>
>  Sent: Friday, July 5, 2013 6:27 AM
>  Subject: [Histonet] periodic acid/h&e
>  
>  
>  Thanks for the tip.  I had some periodic acid so I tried a 2 minute soak in a 0.5% soln of that followed by a tap water wash and then dw then Harris hematoxylin countered with a Eosin-Biebrich scarlet we like on some classical swine fever slides I looked at today. Very nice.  I will play with this more.
>  
>  By the way, We use the hot water/detergent method for all our dewaxing now.
>  
>  We still are using xylene and alcohols for our processing from fixative to paraffin but
>  we will change that eventually.
>  
>  E. Wayne Johnson
>  Enable Ag Tech
>  Beijing
>  
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