[Histonet] periodic acid/h&e
akemiat3377 <@t> yahoo.com
Fri Jul 5 09:38:32 CDT 2013
I forgot to mention the timing. You may try 5 minutes or even 10 minutes before staining in hematoxylin. It will give you even better results. I used 5 minutes in 1 % periodic acid.
Akemi Allison BS, HT(ASCP)HTL
Phoenix Lab Consulting
E-Mail: akemiat3377 <@t> yahoo.com
From: "ewj <@t> pigsqq.org" <ewj <@t> pigsqq.org>
To: histonet <@t> lists.utsouthwestern.edu; """ <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, July 5, 2013 6:27 AM
Subject: [Histonet] periodic acid/h&e
Thanks for the tip. I had some periodic acid so I tried a 2 minute soak in a 0.5% soln of that followed by a tap water wash and then dw then Harris hematoxylin countered with a Eosin-Biebrich scarlet we like on some classical swine fever slides I looked at today. Very nice. I will play with this more.
By the way, We use the hot water/detergent method for all our dewaxing now.
We still are using xylene and alcohols for our processing from fixative to paraffin but
we will change that eventually.
E. Wayne Johnson
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