[Histonet] RE: cytoprep

[Joe W. Walker, Jr.]

I assume that these are conventional Pap tests where the material is smeared onto the slide.  I am curious why the slides are air dried upon collection instead of applying a spray fixative to the slide.  If these are non-gyn slides from a brushing or FNA, then we might need to have a different discussion.

As someone with numerous years experience with cytoprep, I would need more information to help trouble shoot what might be the real issue and to offer suggestions.

Cheers,

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Anatomical Pathology Manager
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN)
Sent: Wednesday, January 30, 2013 5:05 PM
To: 'Nancy Schmitt'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: cytoprep

The saline re-hydration works well as long as smears are not allowed to dry too long before rehydration.
I have found that air-drying longer than 2 hours decreases the effectiveness of the procedure.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt
Sent: Thursday, 31 January 2013 5:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cytoprep

Hi to all-
I am continuing to have a problem with Pap stains done by hand:

1.       We rehydrate 25 seconds in saline and straight into alcohol.  Thicker areas are ok, but thinner areas appear to be air dried......

2.       Red cell ghosts are NOT clearing
I believe there are quite a few Histology labs also performing cytoprep work and I will appreciate any thoughts you may have.

Nancy Schmitt HT, MLT
Dubuque, IA





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